human cr2 protein  (Bio-Techne corporation)

Journal: Analytical Chemistry

Article Title: Early Detection of Multiple Sclerosis Using Spectroscopic Epstein–Barr Virus Biosensors Based on Infection Mechanism Mimicry

doi: 10.1021/acs.analchem.5c03964

Figure Lengend Snippet: SEM (A–C) and AFM (D–F) images of (A, D) CD21@AuNP, (B,E) BSA@CD21@AuNP, and (C, F) gp350@BSA@CD21@AuNP.

Article Snippet: Recombinant human CD21 and recombinant EBV gp350 proteins were purchased from Sino Biological Inc. All reagents and chemicals employed in this study were analytical grade and used without further purification.

Techniques:

Journal: Analytical Chemistry

Article Title: Early Detection of Multiple Sclerosis Using Spectroscopic Epstein–Barr Virus Biosensors Based on Infection Mechanism Mimicry

doi: 10.1021/acs.analchem.5c03964

Figure Lengend Snippet: (A) FTIR and (B) UV spectra for the characterization of the developed colorimetric EBV biosensor. Step-by-step FTIR and UV spectra of the biosensor during gp350 detection: (a) 2.1 mL of AuNPs; (b) 2.1 mL of AuNPs + 400 μL of CD21 at 20 ng·mL –1 ; (c) 2.1 mL of AuNPs + 400 μL of CD21 at 20 ng·mL –1 + 100 μL of 1% BSA; and (d) 2.1 mL of AuNPs + 400 μL of CD21 at 20 ng·mL –1 + 100 μL of 1% BSA + 50 ng·mL –1 of gp350.

Article Snippet: Recombinant human CD21 and recombinant EBV gp350 proteins were purchased from Sino Biological Inc. All reagents and chemicals employed in this study were analytical grade and used without further purification.

Techniques:

Journal: Analytical Chemistry

Article Title: Early Detection of Multiple Sclerosis Using Spectroscopic Epstein–Barr Virus Biosensors Based on Infection Mechanism Mimicry

doi: 10.1021/acs.analchem.5c03964

Figure Lengend Snippet: (A) UV spectra and (B) bar chart that belong to the optimization of the CD21–gp350 interaction incubation temperature. (a) BSA@CD21@AuNP, (b) BSA@CD21@AuNP + 50 ng·mL –1 gp350 for 4 °C, (c) 25 °C, (d) 37 °C, and (e) 45 °C incubation temperature.

Article Snippet: Recombinant human CD21 and recombinant EBV gp350 proteins were purchased from Sino Biological Inc. All reagents and chemicals employed in this study were analytical grade and used without further purification.

Techniques: Incubation

Journal: Analytical Chemistry

Article Title: Early Detection of Multiple Sclerosis Using Spectroscopic Epstein–Barr Virus Biosensors Based on Infection Mechanism Mimicry

doi: 10.1021/acs.analchem.5c03964

Figure Lengend Snippet: (A) UV spectra and (B) Excel plot that belong to the optimization of the CD21–gp350 interaction incubation time. (a) BSA@CD21@AuNP, (b) BSA@CD21@AuNP + 50 ng·mL –1 gp350 for 15 min, (c) 30 min, (d) 60 min, and (e) 90 min incubation time.

Article Snippet: Recombinant human CD21 and recombinant EBV gp350 proteins were purchased from Sino Biological Inc. All reagents and chemicals employed in this study were analytical grade and used without further purification.

Techniques: Incubation

Journal: Analytical Chemistry

Article Title: Early Detection of Multiple Sclerosis Using Spectroscopic Epstein–Barr Virus Biosensors Based on Infection Mechanism Mimicry

doi: 10.1021/acs.analchem.5c03964

Figure Lengend Snippet: Analytical characteristics of the developed colorimetric EBV biosensors. (A) UV spectra of (a) BSA@CD21@AuNP, (b) 10 ng·mL –1 , (c) 25 ng·mL –1 , (d) 50 ng·mL –1 , (e) 80 ng·mL –1 , and (f) 100 ng·mL –1 gp350 additions. (B) Calibration plot of increasing concentrations of gp350 from 10 ng·mL –1 to 100 ng·mL –1 .

Article Snippet: Recombinant human CD21 and recombinant EBV gp350 proteins were purchased from Sino Biological Inc. All reagents and chemicals employed in this study were analytical grade and used without further purification.

Techniques:

Journal: Analytical Chemistry

Article Title: Early Detection of Multiple Sclerosis Using Spectroscopic Epstein–Barr Virus Biosensors Based on Infection Mechanism Mimicry

doi: 10.1021/acs.analchem.5c03964

Figure Lengend Snippet: UV spectra demonstrating the absorbance difference of a 1:1 ratio of (a) BSA@CD21@AuNP, (b) gp350 @BSA@CD21@AuNP, and (c) A27L + KMP11 + gp350 @BSA@CD21@AuNP.

Article Snippet: Recombinant human CD21 and recombinant EBV gp350 proteins were purchased from Sino Biological Inc. All reagents and chemicals employed in this study were analytical grade and used without further purification.

Techniques:

Journal: Frontiers in Immunology

Article Title: Fusion of the molecular adjuvant C3d to cleavage-independent native-like HIV-1 Env trimers improves the elicited antibody response

doi: 10.3389/fimmu.2023.1180959

Figure Lengend Snippet: Activation by complement system, design, and schematic representation of HIV-1 Env-C3d fusion Trimers. (A) Cartoon model depicting enhanced activation of the innate immune system by simultaneous engagement of HIV-1 Env-C3d trimers with naïve B- cell receptors (BCRs) and CR2, CD19, and CD81 receptors of the complement system, respectively. (B) Further activation of the adaptive immune system in germinal centers by the engagement of CD2 receptors on follicular dendritic cells (FDCs) with BCR- attached Env-C3d trimers. (C) Model of Env-C3d (16055 NFL TD CC T569G, PDB 5UM8; human C3d, PDB: 3OED). The three Env gp120 subunits are shown in blue; the gp41 ectodomain subunits in cyan to form the gp140 trimers; mouse C3d in magenta. The three C3d subunits were manually fitted to the C-termini of the NFL trimer by visual inspection in PyMOL. The dotted lines represent the linker connecting the C-termini of NFL Env gp140 to the N-termini of C3d. (D) Linear schematic diagram of JRFL-C3d trimers. Mouse C3d was fused to the C-termini of NFL gp140 trimers by a flexible linker (G 4 S) of varying lengths (14, 30, and 60 amino acids to yield JRFL-L14-C3d, etc.). JRFL NFL TD CC+ (namely, JRFL) was used for the linker-length screening. The native signal peptide sequence of JRFL (or 426c NFL trimers) was replaced by the CD5 leader sequence to increase secretion in mammalian cells, as previously performed.

Article Snippet: For the binding of JRFL-C3d trimers to soluble CR2 protein, his-tagged human CR2 protein (Cat. #10811-H08H, Sino Biological Inc.) was immobilized on anti-HIS sensors.

Techniques: Activation Assay, Sequencing

Journal: Frontiers in Pharmacology

Article Title: Deep Mining of Complex Antibody Phage Pools Generated by Cell Panning Enables Discovery of Rare Antibodies Binding New Targets and Epitopes

doi: 10.3389/fphar.2019.00847

Figure Lengend Snippet: Frequency, based on NGS analysis, of discovered clones in the different strategies including the input phage pool. Median frequency is shown with red bars. (A) Antibodies, previously discovered through direct screening with corresponding specificities, CD23 ( n = 107), CD72 ( n = 132), and CD200, CD21, CD32, or HLA-DR ( n = 34). (B) Antibodies, discovered through fishing, binding FCRL5, ROR1, or CD22 ( n = 17). For statistical analysis, Friedman’s test with Dunn’s multiple comparison was done using GraphPad Prism.

Article Snippet: The extracellular domain of CD72 (R&D Systems, 5405-CD), CD21 (Sino Biological, 10811-H08H), and CD32 (in-house produced) were coupled to Tosylactivated M280 beads (Thermo Fisher Scientific) (200 pmol protein to 60 µl beads), according to manufacturer’s instructions and coated to an immunotube (Thermo Fisher Scientific, 470319), 70 pmol/antigen, overnight.

Techniques: Clone Assay, Binding Assay

Journal: Frontiers in Pharmacology

Article Title: Deep Mining of Complex Antibody Phage Pools Generated by Cell Panning Enables Discovery of Rare Antibodies Binding New Targets and Epitopes

doi: 10.3389/fphar.2019.00847

Figure Lengend Snippet: NGS analysis of identified antibodies binding to (A) CD72 ( n = 301) or (B) CD21 ( n = 27) showing the frequency in control (track C) versus antibody blocking (track B1). Yellow-marked clones were analyzed for binding, as scFv displayed on phage, to coated (C) CD72 or (D) CD21 protein in ELISA with or without prior blocking with the pool of IgGs used for blocking in tracks B1 and B2. As control, two additional clones per target, corresponding to sequences showing a decreased frequency in tracks B1 and B2 in the NGS analysis, were included. Binding was detected using an HRP-labeled anti-M13 antibody and a luminescent substrate.

Article Snippet: The extracellular domain of CD72 (R&D Systems, 5405-CD), CD21 (Sino Biological, 10811-H08H), and CD32 (in-house produced) were coupled to Tosylactivated M280 beads (Thermo Fisher Scientific) (200 pmol protein to 60 µl beads), according to manufacturer’s instructions and coated to an immunotube (Thermo Fisher Scientific, 470319), 70 pmol/antigen, overnight.

Techniques: Binding Assay, Blocking Assay, Clone Assay, Enzyme-linked Immunosorbent Assay, Labeling